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. 2014 Dec 18;6(2):862–873. doi: 10.18632/oncotarget.2696

Figure 2. Siah knockdown induces an EMT gene profile.

Figure 2

(A) The efficiency of Siah knockdown with Dharmacon SMARTpool siRNAs as confirmed by qPCR. (B) mRNA expression of mesenchymal genes, following Siah1 and/or Siah2 knockdown, as assessed by qPCR. (C) The mRNA expression of EMT transcription factors, following Siah1 and/or Siah2 knockdown, as determined by qPCR. (D) Knockdown of Siah1, Siah2 and Zeb1 in MCF-7 cells knockdown with Dharmacon SMARTpool siRNAs. (E) mRNA expression of mesenchymal genes following knockdown of Zeb1 alone, Siah1 and Siah2 or Siah1, Siah2 and Zeb1. (F) The mRNA expression of EMT transcription factors following Zeb1, Siah1 and Siah2, and Siah1, Siah2 and Zeb1 knockdown. All knockdown qPCR reactions were performed in triplicate per biological repeat (n = 5). Gene expression levels are relative to control (set value of 1). Data shown as mean ± SEM.