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. 2014 Nov 25;6(2):902–914. doi: 10.18632/oncotarget.2817

Fig.5. Chemokine receptor expression on OT-I cells.

Fig.5

Splenocytes were harvested from 6-week-old OT-I mice. After overnight culture with RPMI 1640 complete medium containing IL2, cells were doubly stained for chemokine receptors (CXCR2, CCR3 and CCR5) and either CD4 (a) or CD8 (b), or the isotope controls, and analyzed by flow cytometry.