Skip to main content
. 2014 Dec 22;6(3):1422–1434. doi: 10.18632/oncotarget.2805

Figure 3. WNK2 downregulation is associated with increased SRC and JNK activation levels.

Figure 3

Cell lysates were analyzed by Western Blot, immunostained with (A) an anti-phospho Thr202/Tyr204 ERK antibody, and re-stained after stripping of the same membrane for total ERK, and α-tubulin (B) an anti-phospho Thr183/Tyr185 JNK antibody, and re-stained for total JNK, and α-tubulin, after stripping, (C) an anti-phospho Thr180/Tyr182 p38 antibody, and re-stained for total p38, and α-tubulin after stripping of the membrane, (D) an anti-phospho Tyr 416-SRC antibody, and re-stained for total SRC, and α-tubulin after stripping. Graphs correspond to the mean values ± standard errors and are representative of, at least, three independent experiments. *, significantly different from SW1088 C- or A172.Ev cells (p < 0.05), **, significantly different from SW1088 C- or A172.Ev cells (p < 0.01), ***, significantly different from A172.Ev cells (p < 0.001).