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. 2015 Jan 13;6(3):1446–1461. doi: 10.18632/oncotarget.2735

Figure 1. K252a can inhibit linker phosphorylation in mitotic HeLa cells.

Figure 1

(A) Western blot analysis of whole protein extracts from HeLa cells, growing asynchronously (Asy) or arrested with nocodazole (Noc) for 18 hours. The blot was probed with anti-HpTGEKP antibody to visualize the C2H2 ZFP linker phosphor-bands. The blot was then probed with anti-tubulin to show equal loading. A fraction of the cells were tested with FACS analysis for their cell cycle distribution, as a control (right panels) (B) HeLa cells arrested with nocodazole for 18 hours were collected, divided into equal fractions and treated with small-molecule inhibitors, as indicated on the blot, at 1 μM concentration for 10 minutes. Cell lysates were analyzed by Western blotting and probed with anti-HpTGEKP antibody, then with anti-tubulin antibody as loading control.