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. 2015 Jan 20;6(3):1478–1489. doi: 10.18632/oncotarget.2756

Figure 1. Elevated expression of Gab1 is associated with increased activation of mTORCs in urothelial carcinoma.

Figure 1

(A, B) Immunohistochemical staining was performed on paraffin-embedded human urothelial carcinoma tissues and normal urothelial tissues. The sample pictures of immunohistochemical staining of Gab1, mTOR-pS2481 and AKT-pS473 are shown (400X). The lysates from human urothelial carcinoma tissues and normal urothelial tissues were immunoblotted to detect mTOR-pS2448, mTOR-pS2481, p70S6K-pT389, AKT-pS473 and Gab1. N, Normal. T, Tumor. (C, D) Paraffin-embedded tissues from both normal control rats and urothelial carcinoma rats induced by 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) were immunohistochemically stained for Gab1, mTOR-pS2481 and AKT-pS473 (400X). The lysates from normal control rats and rats with urothelial carcinoma were immunoblotted to detect mTOR-pS2448, mTOR-pS2481, p70S6K-pT389, AKT-pS473 and Gab1. N, Normal. T, Tumor.