(A, B) Immunohistochemical staining was performed on paraffin-embedded human urothelial carcinoma tissues and normal urothelial tissues. The sample pictures of immunohistochemical staining of Gab1, mTOR-pS2481 and AKT-pS473 are shown (400X). The lysates from human urothelial carcinoma tissues and normal urothelial tissues were immunoblotted to detect mTOR-pS2448, mTOR-pS2481, p70S6K-pT389, AKT-pS473 and Gab1. N, Normal. T, Tumor. (C, D) Paraffin-embedded tissues from both normal control rats and urothelial carcinoma rats induced by 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) were immunohistochemically stained for Gab1, mTOR-pS2481 and AKT-pS473 (400X). The lysates from normal control rats and rats with urothelial carcinoma were immunoblotted to detect mTOR-pS2448, mTOR-pS2481, p70S6K-pT389, AKT-pS473 and Gab1. N, Normal. T, Tumor.