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. 2015 Jan 10;6(3):1618–1630. doi: 10.18632/oncotarget.2730

Figure 1. The CHD1L downstream target NTKL was frequently overexpressed in HCC.

Figure 1

(A) Two candidate CHD1L binding motifs were found at upstream (–1410~–1400 and –717~–707) of NTKL. The binding of CHD1L to the candidate motif sequences of NTKL was confirmed by ChIP assay. (B) QRT-PCR shows that overexpression of CHD1L in QGY-7703 cells could upregulate NTKL expression. (C) Expressions of NTKL and CHD1L were significantly correlated in 138 primary HCC samples detected by QRT-PCR. (D) NTKL expression was determined by QRT-PCR between tumor and corresponding non-tumor tissues in 138 HCCs, and the fold changes (RQ) was compared using T-test. (E) Expression of NTKL expression in primary HCCs (left) and HCC cell lines (right) was detected by Western blotting. (F) Kaplan-Meier survival analysis of the correlation between NTKL expression and HCC patient overall and disease free survivals.