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. 2015 Jan 10;6(3):1618–1630. doi: 10.18632/oncotarget.2730

Figure 3. Overexpression of NTKL promoted G1/S transition.

Figure 3

(A) Flow cytometry was used to compare cell cycle distribution between NTKL-transfected and Vec-transfected cells. The percentages of cells at G0/G1, S and G2/M were summarized in bar charts. Data was shown as the mean ±SD of three independent experiments (*, P < 0.05; independent Student's t-test). (B) Depletion of NTKL by RNAi blocked cell cycle at G1 phase. Data were shown as the mean ±SD of three independent experiments (*, P < 0.05; independent Student's t-test). (C) The degradation of P53 and CyclinD1 was regulated by NTKL overexpression and depletion. Cells with NTKL overexpression or depletion were treated with CHX (50ug/mL) for time indicated to inhibit protein synthesis, and the degradation of P53 and CyclinD1 was observed by western blot.