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. 2015 Jan 10;6(3):1618–1630. doi: 10.18632/oncotarget.2730

Figure 6. NTKL regulated the reorganization of actin cytoskeleton and interacted with dynamin2.

Figure 6

(A) Representative images showing the morphology of QGY-7703, NTKL-7703 and sh-NTKL-4 cells. The cell areas were measured by image J and results were summarized in the bar chart (*, P < 0.05, **, P < 0.001). (B) Invasive structures (yellow arrows) composed of actin filaments were found in NTKL-7703 cells, but not in QGY-7703 and sh-NTKL-4 cells. Phalloidin staining indicated F-actin organization in QGY-7703, NTKL-7703 and sh-NTKL-4 cells. (C) Reduced p-Rac1/cdc42 was detected in NTKL depleted cells by Western blotting. (D) Results of Co-IP assay using NTKL and IgG (control) antibodies. Proteins recognized by mass spectrometry were indicated. (E) Interaction between NTKL and dynamin2 was confirmed by Co-IP assay using NTKL and dynamin2 antibodies. N, NTKL; D, dynamin2. (F) Colocalization of NTKL and dynamin2 was confirmed by immunofluorescence staining.