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. 2014 Dec 9;6(3):1666–1677. doi: 10.18632/oncotarget.2762

Figure 1. Hypoxia induces Notch ligand expression in malignant tumors.

Figure 1

HSR-GBM1 neurospheres were subjected to normoxia (20% O2) or hypoxia (1% O2) for 1 (T = 1), 6 (T = 6), 9 (T = 9), 22 (T = 22) and 48 (T = 48) h, at which time they were collected for RNA analyses. (A) JAG1 and (B) JAG2 mRNA levels were both induced soon after hypoxia exposure. (C) JAG1 and (D) JAG2 mRNA levels were similarly upregulated in the GBM neurospheres lines JHH-GBM10, JHH-GBM14, 040621 and 040821. (E) JAG1 and NICD protein levels were also induced following 48 h exposure to hypoxia, with JAG1 expression enduring at 72 h. The pancreatic cancer cell line XPA3 also induces JAG1 (F) mRNA and (G) protein levels following 48 h exposure to hypoxia.