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. 2014 Dec 9;6(3):1666–1677. doi: 10.18632/oncotarget.2762

Figure 2. Ectopic ligand expression induces Notch signaling.

Figure 2

(A) Lentiviral transduction of JAG1 resulted in 80% of cells expressing ligand compared to 20% in the control based on three separate immunofluorescence studies (Supplemental Figure 1). The percentage of cells expressing NICD was upregulated approximately 10-fold. Overexpression of JAG1 ligands in (B) HSR-GBM1, (C) 040821 and (D) XPA3 lines upregulates Notch targets from the HES and HEY families. **p < 0.01, ***p < 0.001 compared to pLenti6 control from 3 experiments.