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Notch target levels were reduced in (A) HSR-GBM1 and (B) 040821 cells transduced with ligand ICD. The average sphere diameter of (C) HSR-GBM1 cells transduced with ligand ICDs was reduced in a clonogenic assay. (D) HSR-GBM1 cells transduced with C-terminus DsRed tagged JAG1ICD showed distinct nuclear localization, in distinct contrast to the pLVU/Red vector control transduced cells.
