Table 2. Gateway primers used for designing attB PCR gene products.
Primer | attB1/B2 sequences | Gene specific sequences |
---|---|---|
JAG1ICD_GTW_Forward | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGA AGGAGATAGAACCATGGCG | CGGAAGCGGCGGAAGCCGGGCAGCC |
JAG1ICD_GTW_Reverse | GGGGACCACTTTGTACAAGAAAGCTGGGTC | TACGATGTACTCCATTCGGTTTAAGC |