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. 2015 Feb 11;6(3):1850–1864. doi: 10.18632/oncotarget.2575

Figure 4. Roles of Ras and AKT activation in RasGRP3-induced glioma cell migration.

Figure 4

U87 cells overexpressing CV and RasGRP3 (A) and A172 cells treated with control shRNA or shRNA targeting RasGRP3 (B) were analyzed for the expression and phosphorylation of AKT and ERK1/2 (A,B). Results represent one of three separate experiments that gave similar results. U87 overexpressing CV and RasGRP3 were transfected with H-Ras17N-HA or pcDNA3.1 (control) and the expression of Ras-DN was analyzed using Western blot (C). Transwell migration was performed 72 hrs later, migrating cells were photographed (D), and the number of cells was determined (E). The results represent the means ± S.E of three independent experiments. *P < 0.001. U87 cells overexpressing CV and RasGRP3 were transfected with AKT-KD mutant or pcDNA3.1 (control) and the expression of AKT-KD was analyzed using Western blot (F). Transwell migration was performed 72 hrs later; cells were photographed (G), and their number was determined (H). The results represent the means ± SE of three independent experiments. *P < 0.01