Table 3.
Summary of tagged annotated smORFs
| Localization | Peptidomic evidence | # aa | RPKM | Coverage | TE | Phast Cons | |
|---|---|---|---|---|---|---|---|
| CG32230 | Mitochondrial | Yes | 83 | 539.2 | 1.00 | 3.05 | 0.54 |
| CG14482 | Mitochondrial | Yes | 57 | 600.0 | 1.00 | 1.09 | 0.72 |
| CG44242 | Mitochondrial | Yes | 70 | 152.9 | 0.97 | 1.75 | 0.66 |
| CG7630 | Mitochondrial | Yes | 90 | 702.2 | 1.00 | 1.05 | 0.64 |
| CG33199 | Mitochondrial | No | 79 | 95.5 | 1.00 | 1.17 | 0.59 |
| CG32582 | Mitochondrial | No | 52 | 16.5 | 0.57 | 2.82 | 0.51 |
| sclA | Other cytoplasmic | NA | 28 | NA | NA | NA | NA |
| CG12384 | Other cytoplasmic | Yes | 96 | 205.6 | 1.00 | 1.37 | 0.71 |
| CG33774 | Other cytoplasmic | No | 40 | 115.3 | 1.00 | 1.13 | 0.73 |
| CG33170 | Other cytoplasmic | No | 71 | 84.2 | 0.84 | 0.75 | 0.60 |
| CG34200 | Limited | Yes | 52 | 331.7 | 1.00 | 1.66 | 0.54 |
| CG32267 | Limited | Yes | 49 | 82.5 | 0.97 | 1.13 | 0.70 |
| CG33155 | Limited | No | 60 | 33.8 | 0.64 | 0.88 | 0.67 |
| tal-B | None | NA | 49 | NA | NA | NA | NA |
Details of the Poly-Ribo-Seq and transfection translation assay results for the FLAG-tagged smORFs, with RPKM, coverage and TE values. Previously corroborated smORFs (according to Figure 1A) are in bold. Scl is a positive control and tal-B is a negative control, but both are not endogenously transcribed in S2 cells, hence ‘NA’ Polysomal Ribo-Seq metrics and Peptidomic evidence.