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. 2015 Feb 25;8:125. doi: 10.1186/s13071-015-0728-2

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© Lin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression, purification and characterization of Cs ACAT. (A) rCsACAT was identified by 12% SDS-PAGE. Protein molecular weight markers (lane M), lysate of E. coli containing pET28a (+) without induction (lane 1) and with induction by IPTG (lane 2), lysate of E. coli containing pET28a (+)-CsACAT without induction (lane 3) and with induction by IPTG (lane 4), supernatant (lane 5) and precipitant (lane 6) of lysate of E. coli containing the recombinant plasmid after induction, the purified recombinant CsACAT protein (lane 7). (B) Western blotting analysis of CsACAT. Lane 1–4, rCsACAT probed with naive serum, anti-His tag monoclonal antibody, rat anti-rCsACAT serum and serum from C. sinensis-infected rat; lane 5–6, CsESPs probed with naive serum and rat anti-rCsACAT serum; lane 7–8, total proteins of adult worm probed with naive serum and rat anti-rCsACAT serum; lane 9, purified rCsACAT blotted with rat anti-CsESPs serum.