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. 2015 Feb 25;8:125. doi: 10.1186/s13071-015-0728-2

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© Lin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression pattern of Cs ACAT. (A) Immunolocalization of CsACAT in C. sinensis adult. Panel a and b, sections treated with anti-rCsACAT serum and specific fluorescences distributed in the vitellarium and sub-tegumental muscular layer of the adult worm; Panel c and d, sections treated with naive serum. No fluorescence was detected. M, sub-tegumental muscular layer; V, vitellarium. Magnification for the adult worm was × 100. (B) mRNA level of CsACAT at different developmental stages of C. sinensis by quantitative real-time PCR. The specific mRNA fragment of CsACAT was observed among the stages. The quantities were normalized with Cs β-actin and analyzed by means of the 2^−ΔΔCt ratio.