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. 2015 Feb 25;8:125. doi: 10.1186/s13071-015-0728-2

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© Lin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Worm burden in rabbit models and expression level of Cs ACAT in the worms. (A) Worm burden in hypercholesteremia or basal diet fed rabbits 6 weeks after C. sinensis infection. All data are presented as mean ± SD, *p < 0.05, **p < 0.01. (B) The mRNA level of CsACAT in adult worms extracted from hypercholesteremia or basal diet fed rabbits, normalized with Cs β-actin. (C) The protein levels of CsACAT in adult worms of the two groups. Lane 1–3, total proteins of worms recovered from basal diet treated rabbits probed with rat anti-rCsACAT serum. Lane 4–6, total proteins of worms recovered from hypercholesteroemic rabbits blotted with rat anti-rCsACAT serum. (D) Optical density analysis of the protein levels in Figure 5C. The optical density was calculated by Tanon Gis software and analyzed by Student’s t test, *p < 0.05.