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. 2015 Feb 25;8:125. doi: 10.1186/s13071-015-0728-2

Table 1.

Enzymatic activity of Cs ACAT in adult worms incubated with different concentrations of lecithin

Fraction Concentration (μg/μl) Volume (μl) Total protein (μg) Total activity units (μmol/min) Specific activity units/mg protein Relative activity (%)
Naïve ACAT activity# 6.68 5 33.41 0.024 0.729 100
6 mM lecithin* 9.82 5 49.09 0.014 0.293 40.19
0.6 mM lecithin* 11.03 5 55.15 0.01 0.181 24.81
0.06 mM lecithin* 12.99 5 64.95 0.011 0.168 23.03
Control** 11.12 5 55.58 0.006 0.104 14.31

The crude extracts of worms incubated with different conditions were collected at 48 h. #, Enzymatic activity of CsACAT in worms newly recovered from liver of infected rat was set as 100%; *, Lecithin was firstly dissolved with 100% alcohol in a 56°C water bath and then diluted with PBS to prepare the mixture containing different concentrations of lecithin; **, Worms cultured with 0.6% alcohol in PBS was applied as a negative control.