Table 1.
Fraction | Concentration (μg/μl) | Volume (μl) | Total protein (μg) | Total activity units (μmol/min) | Specific activity units/mg protein | Relative activity (%) |
---|---|---|---|---|---|---|
Naïve ACAT activity# | 6.68 | 5 | 33.41 | 0.024 | 0.729 | 100 |
6 mM lecithin* | 9.82 | 5 | 49.09 | 0.014 | 0.293 | 40.19 |
0.6 mM lecithin* | 11.03 | 5 | 55.15 | 0.01 | 0.181 | 24.81 |
0.06 mM lecithin* | 12.99 | 5 | 64.95 | 0.011 | 0.168 | 23.03 |
Control** | 11.12 | 5 | 55.58 | 0.006 | 0.104 | 14.31 |
The crude extracts of worms incubated with different conditions were collected at 48 h. #, Enzymatic activity of CsACAT in worms newly recovered from liver of infected rat was set as 100%; *, Lecithin was firstly dissolved with 100% alcohol in a 56°C water bath and then diluted with PBS to prepare the mixture containing different concentrations of lecithin; **, Worms cultured with 0.6% alcohol in PBS was applied as a negative control.