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. 2015 Feb 21;14:47. doi: 10.1186/s12943-015-0320-6

Figure 2.

Figure 2

Involvement of KLF4 in ITLN1-mediated up-regulation of NDRG2. (A) Scheme of the potential binding site of KLF4 within NDRG2 promoter. (B) Luciferase reporter assay showing the activity of NDRG2 promoter and its mutant in NB cells stably transfected with empty vector (mock), ITLN1, sh-Scb, or sh-ITLN1, and those co-transfected with sh-KLF4 or KLF4. (C) ChIP and qPCR assays showing the binding of KLF4 on −133/+55 region of NDRG2 promoter in NB cells stably transfected with mock, ITLN1, sh-Scb, or sh-ITLN1, and co-transfected with sh-KLF4 or KLF4. (D) Western blot showing the protein levels of ITLN1 [in culture supernatant (s)], KLF4, and NDRG2 (in lysate) in NB cells stably transfected with mock, ITLN1, sh-Scb, or sh-ITLN1, and those co-transfected with sh-KLF4 or KLF4. (E) The transcript levels of ITLN1, KLF4, and NDRG2 in NB cells stably transfected with mock, ITLN1, sh-Scb, or sh-ITLN1, and those co-transfected with sh-KLF4 or KLF4 as detected by real-time quantitative RT-PCR. *P < 0.01 vs. mock or sh-Scb.