Figure 5.

ITLN1 attenuates the growth and metastasis of NB cells in vivo . (A and D) Tumor growth curve of SH-SY5Y cells (1 × 10⁶) stably transfected with empty vector (mock), ITLN1, sh-Scb, or sh-ITLN1 in athymic nude mice (n = 5 for each group), after hypodermic injection for 4 weeks. (B and E) Representation (top) and quantification (bottom) of xenograft tumors formed by hypodermic injection of SH-SY5Y cells stably transfected with mock, ITLN1, sh-Scb, or sh-ITLN1. (C and F) Representation (top, arrowhead) and quantification (bottom) of lung metastasis after injection of SH-SY5Y cells (0.4 × 10⁶) stably transfected with mock, ITLN1, sh-Scb, or sh-ITLN1 into the tail vein of athymic nude mice (n = 5 for each group). **P < 0.001 vs. mock or sh-Scb.