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. 2015 Mar 4;12:38. doi: 10.1186/s12985-015-0263-y

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© Ammara et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Southern blot analysis of transgenic tomato plants harboring the hpRNAi construct inoculated with TYLCV-OM probed for the presence of TYLCV-OM (A) and inoculated with TYLCV-OM/ToLCB-OM probed for the presence of ToLCB-OM (B). The DNA samples run on the gel were extracted from a non-transgenic non-inoculated tomato cv. Pusa Ruby plant (N), symptomatic TYLCV-OM (panel A) or TYLCV-OM/ToLCB-OM (panel B) inoculated non-transgenic Pusa Ruby plants (C) and two plants each of 9 transgenic Pusa Ruby lines (as indicated on the figure) inoculated with either TYLCV-OM (panel A) or TYLCV-OM/ToLCB-OM (panel B). Approx. 10 μg of total DNA was loaded on each case and the samples were extracted at 60 dpi. The positions of the viral single-stranded (ss) and supercoiled (sc) replicative DNA forms are indicated.