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. 2015 Feb 28;8:132. doi: 10.1186/s13071-015-0749-x

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© Sultana et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of O. turicata subolesin is developmentally regulated. A) Total RNA from unfed larvae, nymphs and female adults was prepared and amount of O. turicata subolesin transcripts were quantified by QRT-PCR normalized to O. turicata 28S levels. Each triangle/square/circle represents one individual tick. B) Expression of subolesin transcripts in salivary glands (SG) and midguts (MG) isolated from fed adult female O. turicata ticks are shown. Each inverted triangle/circle represent one individual tissues sample isolated from one tick. C) QRT-PCR analysis of subolesin mRNA levels in fed O. turicata female ticks at different days post-feeding is shown. Each circle represents one tick. Statistics was performed using the Student’s t test and the P value is shown.