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. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7

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© Holehonnur et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Vector maps of AAV vectors optimized for large transgenes. Viral genome maps for pAAV vector backbones containing either the Flag-GluN2A/B or GFP transgenes. Each of these vectors contain a ~75 bps 3′UTR that contains an SV40 based poly-adenylation signal. These transgenes are controlled by one of the following promoters as indicated: 1.3αCaMKII, 0.4αCaMKII, 1.1Synapsin, 0.5Synapsin, CMV, TRE3G, EFS, or RSV. The promoters are depicted as their approximate relative sizes to one another. GFP = green fluorescence protein, ITR = inverted terminal repeat, MCS = multiple cloning site.