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. 2015 Mar 8;16:4. doi: 10.1186/s12867-015-0035-7

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© Yang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Validating SFRP1 as a positive target for miRNA-1 and miRNA-206. Cotransfection of porcine pre-miRNA-1 (A) and pre-miRNA-206 (B) or control and porcine SFRP1 UTR-derived psiCHECK-2 construct or mutant in PIEC cells. Renilla activity at 48 h post-transfection shows a significant decrease in normalized values compared with the control and mutant. Three replicates were performed for each group. **Indicates a p-value of less than 0.01 in Student’s t-test.