Table 3.
Number of chickens positive for influenza A virus in oropharyngeal (OP) and cloacal (CL) swabs by rRT-PCR or virus isolation by day post inoculation (DPI) and number of chickens positive by hemagglutination inhibiton assay for antibody to A/Anhui/1/2103 H7N9 at 10 or 14 (DPI). There were no significant differences between the number of white leghorns and white rocks shedding except in the 108EID50/bird dose group inoculated intrachoanally at 10DPI by the oral route (*).
| Dose | Route of Inoculation |
Breed | 2 DPI | 4 DPI | 6 DPI | 8 DPI | 10 DPI | Serology | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| OP | CL | OP | CL | OP | CL | OP | CL | OP | CL | # Pos/ Total (% pos) |
GMTc | |||
| 106EID50/bird | Intrachoanal | White Leghorns | 1/5 (20)a | Not done | 3/5 (60) | Not done | 3/5 (60)b | Not done | Not done | Not done | Not done | Not done | 3/5 (60) | 40 |
| Intrachoanal | White Plymouth Rocks | 8/13 (61.5) | Not done | 6/10 (60) | Not done | 7/10 (70)b | Not done | Not done | Not done | Not done | Not done | 7/10 (70) | 18 | |
| 108EID50/bird | Intrachoanal | White Leghorns | 14/14 (100) | 2/14 (12.5) | 12/12 (100) | 2/12 (16.7) | 11/12 (91.6) | 3/12 (25) | 4/9 (44.4) | 2/9 (22.2) | 5/9* (55.5) | 2/9 (22.2) | 9/9 (100) | 102 |
| Intrachoanal | White Plymouth Rocks | 10/10 (100) | 1/10 (10) | 8/8 (100) | 3/8 (37.5) | 8/8 (100) | 2/8 (25) | 2/6 (33.3) | 1/6 (16.7) | 0/6* (0) | 0/6 (0) | 6/6 (100) | 66 | |
| 108EID50/bird | Intrachoanal | White Leghorns | 6/6 (100) | 2/6 (33.3) | 6/6 (100) | 6/6 (100) | Not done | Not done | Not done | Not done | Not done | Not done | 4/4 (100) | 256 |
| Intravenous | White Leghorns | 6/6 (100) | 6/6 (100) | 6/6 (100) | 6/6 (100) | Not done | Not done | Not done | Not done | Not done | Not done | 4/4 (100) | 724 | |
a
Number positive/total (percent).
b
Samples collected 7DPI.
c
GMT=Geometric mean titer