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Protein lysates from cultured human fetal neurons (HFN) at DIV 17 were subjected to an in vitro calpain cleavage assay as described above. Immunoblot analysis of resulting samples assessed levels of E2F1 and calpain-cleaved αII-spectrin to measure calpain activation. Lysate from NMDA-treated rat cortical cultures (RCN) served as a positive control for calpain-cleaved E2F1.
