Fig. 4.
VIP-Fos colocalization (% of VIP neurons co-expressing Fos) increases in the BSTm of nesting males and correlates with the amount of time that males spend in the nest cup. (A) ANOVA shows a trend for a Sex*Condition interaction for VIP-Fos colocalization in the BSTm, with male nesting subjects (solid bar) showing greater colocalization than males that were not given nesting material (open bar). This difference is confirmed in a pairwise comparison (different letters above the error bars indicate p < 0.05; unpaired t-test). Nesting subjects are shown in solid bars. (B) VIP-Fos colocalization correlates positively with time in the nest cup for all males. Data points from nesting subjects are shown in heavy circles; data points from subjects with nest cups only are shown in lighter circles.