Table 2.
PCR conditions for bla genes amplification
| No. Cycles | Final extension Temperature (time) | Extension Temperature (time) | Annealing Temperature (time) |
Denaturation
Temperature (time) |
Initial denaturation Temperature (time) | Gene |
|---|---|---|---|---|---|---|
| 30 | 72 oC (7 min) | 72 oC (1 min) | 60 oC (40 sec) | 94 oC (40 sec) | 94 oC (10 min) | bla VIM & blaIM: |
| 30 | 72 oC (7 min) | 72 oC (1 min) | 55 oC (1 min) | 94 oC (30 sec) | 94 oC (1 min) |
bla
OXA-23 & blaOXA-48 |
| 30 | 72 oC (1 min) | 72 oC (1 min) | 60 oC (40 sec) | 94 oC (30 sec) | 94 oC (1 min) |
bla
NDM-1 & blaSPM-1 |
| 30 | 72 oC (5 min) | 72 oC (1 min) | 56 oC (1 min) | 94 oC (1 min) | 94 oC (5 min) | bla KPC |
| 30 | 72 oC (5 min) | 72 oC (1 min) | 58 oC (1 min) | 94 oC (45 sec) | 94 oC (5 min) | bla OXA-51 |