Fig. 2.

Quantitative real-time RT-PCR detection of manA gene expression and specific enzyme activity of phosphomannose isomerase (PMI) in the transgenic lines (tp-29, tp-13, and tp-7) and non-transgenic control plants from seed-cane (wt) and tissue-cultured (tc) plants. The manA gene was considered differentially expressed in this article if its relative expression was twofold or greater. No enzyme activity was detected in the non-transgenic control plants from seed-cane (wt) and tissue-cultured (tc) plants