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(A) Scheme of Flag-Plk4 fragments. (B) After overexpression of Flag-Plk4-fragments (A) and GFP-STIL in HEK293T cells, cell lysates were subjected to immunoprecipitations using anti-Flag antibodies. Coprecipitation of GFP-STIL with Flag-Plk4 fragments was detected by western blotting using anti-GFP and anti-Flag antibodies.
