Table 1.
Target gene | Primer sequences |
---|---|
GAPDH: | F5′-GTG AAG GTC GGA GTC AAC GG-3′ |
R5′-CCA TCA CGC CAC AGT TTC CC-3′ | |
| |
P450arom: | F5′-CAG AGT ATC CGG AGG TGG AA-3′ |
R5′-ACT CGA GCC TGT GCA TTC TT-3′ | |
| |
COX-2: | F5′-AAA GCC TCG TCA GAT GCT A-3′ |
R5′-ATG GTG GCT GTC TTG GTA GG-3′ | |
| |
17β-HSD-1: | F5′-GTT ATG AGC AAG CCC TGA GC-3′ |
R5′-TCT GGA TCC CCT GAA ACT TG-3 | |
| |
17β-HSD-2: | F5′-GCC CTG GTG CTC TAG AAC TG-3′ |
R5′-AGT TCC ACA TCG GCC ACT AC-3′ |