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. 2015 Mar 15;128(6):1139–1149. doi: 10.1242/jcs.163063

Fig. 2.

Fig. 2.

Fz4 colocalizes with ADAM13 in CNC cells. ADAM13-GFP, Fz4-RFP and Fz4-v1 RFP were expressed in CNC explants placed on fibronectin substrate. The fluorescence was detected using a spinning disc confocal acquiring every 2 seconds for one minute. Frames from each movie were extracted and annotated. ADAM13-GFP colocalizes with Fz4-RFP in vesicles (boxed areas and arrowhead) 10 µm from the fibronectin substrate (upper panel). At the plasma membrane in contact with the substrate, ADAM13-GFP colocalizes with Fz4-RFP in both vesicles (arrowheads) and at the membrane (arrow, middle panel). ADAM13-GFP is detected in close association with Fz4-v1 in membrane protrusions in direct contact with the fibronectin substrate (boxed areas, lower panel).