Fig. 7.

Overexpression of ADAM13 reduces the severity of the CNC-positioning defect caused by loss of Fz4. (A,B) In situ hybridization with Sox10 and Twist to label neural crest cells in embryos injected with various constructs. Representative examples of the various phenotypes are on the left and are color-coded. Asterisks indicate the injected side. The histogram on the right represents the percentage of phenotypes observed for each injection. The number of embryos (n) is indicated at the top of each bar. Gray, wild type; yellow, a mild inhibition; orange, embryos with a more-dorsal position of the CNC and a less defined segment (fused); red, embryos in which the CNC cells have migrated less than half the distance from the control side (strong inhibition). (A) Embryos were injected at the 4-cell stage. Similar results were obtained by injecting one cell at the 8-cell stage (B), similar to the targeted injections. Scale bars: 500 µm.