Fig. 8.

Myosin X interacts with βarr2. (A) GST–βarr2 pulldown of HEK cells expressing MYO10–GFP demonstrates that myosin X interacts with βarr2. The upper panel shows Coomassie staining; the lower panel shows immunoblotting (IB) for GFP. (B) GST–βarr2 pulldown from mouse brain lysates identifies endogenous myosin X as a βarr2-interacting protein. The upper panel shows Coomassie staining; the lower panel shows immunoblotting. As a positive and negative control for the βarr2 interaction, β-adaptin and actin were probed for, respectively. (C) Cells were transiently co-transfected with 834del–Lgr5 along with the constructs indicated and were stimulated with isoproterenol. The FLAG epitope was immunoprecipitated (IP) to pull down β-arr2–FLAG, and the blots were probed with GFP-, FLAG- or GAPDH-specific antibodies.