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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Int J Biochem Cell Biol. 2015 Jan 23;61:29–34. doi: 10.1016/j.biocel.2015.01.012

Fig. 3. MAGI-1 PDZ3 is required for CAR-mediated enrichment of MAGI-1 at cell junctions.

Fig. 3

COS-7 cells were transfected with A) MAGI-1, B) CAR and MAGI-1, C) CAR and MAGI-1 with the PDZ1 domain deleted, or D) CAR and MAGI-1 with the PDZ3 domain deleted, and immunostained with an anti-CAR Ab (1605p; red) and anti-HA Ab (directed against MAGI-1; green). Nuclei are stained with DAPI (blue). 60 x oil immersion confocal microscopy. Single X-Y optical sections are shown. Merge of optical sections shown in final panel. White arrow identifies a representative junction between two adjacent cells taken from 3 replicate experiments. White bar = 10 μm.