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. Author manuscript; available in PMC: 2016 Mar 15.
Published in final edited form as: Cancer Res. 2015 Jan 9;75(6):1046–1055. doi: 10.1158/0008-5472.CAN-14-1851

Fig. 3.

Fig. 3

ATG7 and BECN1 knockdown causes IRF1 nuclear localization. A, LCC1 cells transfected with IRF1, ATG7, BECN1, or Ctrl siRNA were used to determine IRF1 and ERα localization by immunofluorescent confocal microscopy. B-C, LCC1 and LCC9 cells with transfected with Ctrl, ATG7 (B) or BECN1 siRNA (C) and treated with 100 nM ICI for 48 hours. Western blot hybridization of protein homogenates was used to measure IRF1, ATG7, BECN1, STAT1, P-STAT1, and BCL2. D, MDA-MB-231 cells were transfected with ERα or control cDNA and treated with vehicle or 100 nM ICI for 48 hours. Western blot hybridization of protein homogenates was used to measure ERα and IRF1 expression. E, Western blot images of LCC1 cells transfected with Ctrl, ATG7, STAT1, ATG7+STAT1 siRNA and treated with vehicle 100 nM ICI, or 15 nM JAK inhibitor (JI1) for 48 hours. β-actin served as the loading control. n = 3 independent experiments.