Fig. 5.

Mathematical modeling of proliferation data and experimental validation. A, LCC1 cells transfected with IRF1 or Ctrl siRNA were treated with vehicle control or ICI for 6 days and cell density measured by crystal violet. B, LCC1 cells transfected with Ctrl, IRF1, ATG7, or IRF1+ATG7 siRNA were treated with vehicle control or 100 nM ICI for 6 days before analysis by crystal violet assay. Relative cell densities of each case are plotted. Western blot analysis shows each knockdown target of interest. n = 3 independent experiments; *P < 0.05, ***P < 0.001 versus indicated groups. C, Wiring diagram of model. D, Model predictions for increasing the concentration of siATG7 by a factor of 2. E, Predictions for increasing the concentration of siIRF1 by a factor of 3. F, Experimental results for increasing the concentration of siIRF1 by a factor of 3. n = 3 independent experiments; ***P < 0.001 versus indicated groups