Figure 4. CSF1R blockade effectively lowered tumoral and systemic macrophage levels.

Myc-CaP tumor bearing mice received surgical castration or sham surgery when tumors reached 300–500 mm³, and then fed with control or PLX3397 chow daily for 36 days. A) Representative images of tumor sections stained (left panel) with anti-phosphorylated CSF1R (CSF1R-Tyr723) from 4 treatment groups. Scaled bars represent 100 μm. The right graph shows the quantification of IHC staining. B) Representative flow cytometry plots of B) total CD11B⁺CSF1R⁺ TAMs (left) in tumors, and C) total CD11B⁺CSF1R⁺ myeloid cells in peripheral blood, along with quantification of each (right). *P < 0.05. (n = 6–10 per group).