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. 2015 Jan 21;308(6):F650–F660. doi: 10.1152/ajprenal.00516.2014

Fig. 1.

Fig. 1.

Glycogen synthase kinase (GSK)3α and GSK3β are expressed in adult mouse collecting ducts. A and B: immunofluorescence staining for GSK3α and GSK3β in wild-type (WT) C57Bl/6J mouse kidneys. A: aquaporin 2 (AQP2; green) and Dolichos biflorus agglutinin (DBA; red) staining represent collecting ducts. B: Tamm-Horsfall glycoprotein (THP; green) staining represents the thick ascending limb (TAL). C: GSK3α and GSK3β mRNA relative to 18S. D: Western blot analysis for GSK3α and GSK3β in isolated renal inner medullary collecting ducts (IMCDs). n = 6. *P < 0.05 compared with the cortex.