Fig. 2.

GSK3α knockout (GSK3αKO) mice have polyuria and reduced urine concentrating ability. A: Western blot analysis showing no GSK3α protein in tissue lysates of the medulla and cortex of GSK3αKO mice. GSK3β levels increased in the medulla of GSK3αKO mice compared with WT mice. B: hematoxylin and eosin staining showed no gross morphological abnormalities in GSK3αKO kidneys. C–G: GSK3αKO mice showed increased water intake (C) and urine output (D), reduced urine osmolality (E), and increased urine vasopressin (F) and PGE₂ (G) compared with WT mice. n = 8 mice/group. **P < 0.01 and ***P < 0.0001 compared with WT mice.