Fig. 3.

AQP2 abundance is reduced in GSK3αKO mice. Renal medullary AQP2 and phosphorylated (p)AQP2 (pSer²⁵⁶ AQP2) levels were reduced in GSK3αKO mice, as demonstrated by Western blot analysis (A) and quantitation of band density (B). C: AQP2 mRNA relative to β-actin was reduced in GSK3αKO mice. n = 6 mice/group. D: immunostaining showing reduced AQP2 and pAQP2 staining in the renal papilla of GSK3αKO mice. E: in the cortico-medullary junction of GSK3αKO kidneys, many collecting ducts showed very limited AQP2 (green; inset). Collecting ducts with normal AQP2 expression could also be seen in the same area (*). Magnification: ×63. *P < 0.05 compared with WT mice.