Fig. 3.

Effect of Ca²⁺ and inhibitors of Ca²⁺ signaling molecules on flow-regulated ET-1 mRNA in IMCD3 cells. Cells were preincubated with perfusate HBSS, Ca²⁺-free media, or 50 μM BAPTA-AM (an intercellular Ca²⁺ chelator) (A); 20 μM calmidazolium chloride [an inhibitor of calmodulin (CaM)] or 10 μM KN-93 [a CaM-dependent kinase (CaMK) inhibitor] (B); 0.1 μM calphostin C (CalC; a PKC inhibitor) or 2 μM U-71322 [a phospholipase C (PLC) inhibitor] (C); and 3 μg/ml cyclosporine A (CyA) or 10 μM calcineurin inhibitory peptide (CiP) (both inhibitors of calcineurin) (D) for 30 min. Cells were then subjected to static or flow (2 h at 2 dyn/cm²) conditions followed by the determination of ET-1/GAPDH mRNA levels. n = 12 for each data point. *P < 0.05 vs. cells treated identically but not exposed to flow; #P < 0.05 vs. the no-flow control.