Fig. 4.

Effect of transient receptor potential (TRP) channel inhibition or knockdown on flow-stimulated ET-1 mRNA levels in IMCD3 cells. For inhibitor experiments, cells were pretreated for 30 min with 10 μM Pyr3 (a TRPC3 inhibitor), 20 μM SKF-96365 (SKF; a TRPC6 inhibitor), or 30 μM RN-1734 (a TRPV4 inhibitor). To study the role of polycystin-2, IMCD3 cells with TRPP2 or TRPP2 knockdown were used. Cells were exposed to static or flow (2 h at 2 dyn/cm²) conditions followed by the determination of ET-1/GAPDH mRNA levels. n = 12 for each data point. *P < 0.05 vs. cells treated identically but not exposed to flow.