Fig. 12.

Effects of estrogen on NaPi-IIa protein abundance in U20S cells expressing ERα, ERβ, or ERα and ERβ and transfected with rat NaPi-IIa construct. U20S cells were pretreated with doxycycline and transfected with rat NaPi-IIa full-length construct and then treated with E₂ (100 nM) or vehicle for 24 h. The Immunoblots show the abundance of NaPi-IIa and actin proteins (A, C, E) and the corresponding densitometric analysis of NaPi-IIa/actin (B, D, F) in cells expressing ERα alone (A, B) or ERβ alone (C, D) or both ERα and ERβ (E, F). Each lane is loaded with 30 μg of protein harvested from a different cell culture dish. Five different dishes were used for each group and in each experiment.