Fig. 3.

Expression of ERα and ERβ in the kidney proximal tubule. Kidneys were harvested from OVX rats, and total RNA was isolated from the cortex or proximal tubular suspension (PTS). RNA was reverse transcribed, and resulting cDNA was PCR amplified using specific primers for ERα (A, top) or ERβ (A: bottom). Specific markers of proximal tubule cells SGLT1 (B, top) and SNAT3 (B: bottom) and distal tubule cells NCX (C, top) and ECaC1 (C, bottom) were used to evaluate the enrichment of proximal tubule fragments in the tubular suspensions. GAPDH is used as a control for cDNA loading. In negative samples, the reverse transcriptase was omitted in the reverse transcription reaction (RT). L: 1 kb Plus DNA ladder. The ladders for GAPDH in A and for SNAT3 in B were rearranged to maintain consistency in the position of the ladder in the entire graph.