Fig. 6.

Effect of ERα agonist (PPT) on NaPi-IIa protein expression in the kidney cortex. Total membrane proteins were isolated from vehicle and PPT-treated OVX rats and used for immunoblotting experiments. A: immunoblots of NaPi-IIa and actin proteins in the cortex of rats treated with PPT at 150 μg/100 g body wt (top) or 500 μg/100 g body wt (bottom) vs. pair-fed vehicle. B: corresponding densitometric analysis of NaPi-IIa/actin bands for each dose of PPT vs. vehicle-PF (0). Each lane was loaded with 40 μg of proteins harvested from a different rat kidney cortex. The PPT experiment at 150 μg/100 g body wt was repeated twice (n = 8 rats in each group). n = 4 rats in each group were used for the experiment of PPT at 500 μg/100 g body wt.