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. 2015 Jan 21;308(6):F522–F534. doi: 10.1152/ajprenal.00386.2014

Fig. 8.

Fig. 8.

Effect of ERβ agonist (DPN) on NaPi-IIa expression in the kidney cortex. Total membrane proteins were isolated from vehicle and DPN-treated OVX rats and used for immunoblotting experiments. A: immunoblots of NaPi-IIa and actin proteins in the cortex of rats treated with DPN at 150 μg/100 g body wt (top) or 500 μg/100 g body wt (bottom) vs. vehicle. B: corresponding densitometric analysis of NaPi-IIa/actin bands for each dose of DPN vs. vehicle (0). Each lane was loaded with 40 μg proteins harvested from a kidney cortex of a different rat. The DPN experiment at 150 μg/100 g body wt was repeated twice (n = 8 rats in each group). n = 4 rats in each group were used for the experiment of DPN at 500 μg/100 g body wt. P < 0.02 vs. vehicle (0).