Fig. 9.
Effects of combined ERα and ERβ agonists (PPT + DPN) on food intake, Pi excretion, and NaPi-IIa expression. OVX rats were placed in metabolic cages and injected with PPT + DPN (150 μg/100 g body wt each) or vehicle. Averages of food intake (A) and Pi/creatinine excretion (B) of the last day of treatment with Vehicle or PPT + DPN. C: Northern hybridization of NaPi-IIa and GAPDH mRNA. E: immunoblots of NaPi-IIa and actin proteins in the cortex of rats treated with PPT + DPN vs. vehicle. Corresponding densitometric analysis of NaPi-IIa mRNA/GAPDH mRNA (D) and NaPi-IIa protein/actin (F). Each lane was loaded with 30 μg of total RNA or 40 μg proteins harvested from a different rat. n = 3 or 4 rats in each group. NS, not significant.