Fig. 3.
MitoQ does not affect the basal O2 consumption rate (OCR) in isolated adult cardiomyocytes. Neither MitoQ nor (decyl)triphenylphosponium (dTPP), the targeting scaffold of MitoQ, changed basal or mitochondrial function in control rat cardiomyocytes. Left: OCR plotted over time. Right: quantification. MitoQ or dTPP (50 and 100 nM) were added at 20 min. Oligomycin (O; 1 μg/ml) was used to determine non-ATP-dependent O2 consumption by inhibiting complex V of the mitochondria. FCCP (F; 1 μM) was used to uncouple mitochondrial respiration and stimulate the maximal OCR. Rotenone/antimycin A (R/A; 1 μM/10 μM) was used to completely inhibit the mitochondrial electron transport chain, indicating the nonmitochondrial OCR. n = 6–7 cells/group.