Fig. 5.

MitoQ decreases oxidative stress and protects mitochondrial membrane potential in cardiomyocytes isolated from 8-wk ACF rats. Analysis of ROS by 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein (CM-DCF), a fluorescent marker of oxidative stress, in cardiomyocytes isolated from 8-wk ACF rat hearts indicated a significant increase in ROS in ACF rats, which was attenuated by treatment with MitoQ (top). Mitochondrial membrane potential, as measured by TMRM, was significantly depolarized in ACF animals and improved by treatment with MitoQ (bottom). n = 50 cells/group. *P < 0.05 vs. the sham group.